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Boost Dairy Production and Cut Emissions: New Insights on 3-NOP and Tannin Use in Cows

Learn how 3-NOP and tannins can boost milk production and cut emissions. Ready to improve your herd’s performance? Read more.

Summary: The dairy industry is struggling to balance high milk output with sustainability as regulatory organizations impose stricter limits on methane emissions and nitrogen excretion. 3-nitrooxypropanol (3-NOP) is an innovative feed additive that lowers methane emissions by blocking an enzyme required for methane synthesis in microorganisms, thus improving cow digestion and energy utilization for milk production. Research indicates that cows on a 3-NOP-supplemented diet may reduce methane emissions by 16% to 17% while maintaining milk output. The combination of 3-NOP and tannins has the potential to significantly enhance the dairy industry’s feed efficiency and methane emission reduction efforts.

  • 3-NOP supplementation led to a significant reduction in methane emissions by 16-17%.
  • Brown Swiss and Holstein Friesian cows responded differently to 3-NOP, with Holsteins showing a more significant reduction in methane production.
  • Tannins did not affect milk yield but reduced urinary nitrogen while increasing fecal nitrogen, suggesting better nitrogen utilization.
  • No adverse effects on feed efficiency were observed for 3-NOP or tannin treatments.
  • Combined supplementation of 3-NOP and tannins could offer dual methane mitigation benefits and improved nitrogen management.
  • The study highlights the necessity for further research to optimize additive use and understand breed-specific responses.
dairy industry, high milk output, sustainability, methane emissions, nitrogen excretion, 3-nitrooxypropanol, feed additive, enzyme, microorganisms, cow digestion, energy flow, milk production, environment, farm, research, 3-NOP-supplemented diet, tannins, Acacia mearnsii, naturally occurring chemicals, protein precipitation, nitrogen control, feed efficiency

Are you seeking solutions to increase dairy farm output while lowering hazardous emissions? In today’s world, dairy producers must balance growing milk output with reducing their environmental impact. It’s a delicate balance, but the current study on 3-nitrooxypropanol (3-NOP; Bovaer ®10) and tannin extract (Acacia mearnsii) holds great promise for those prepared to try new things. Imagine the potential of simultaneously improving breastfeeding performance, reducing methane emissions, and optimizing nitrogen utilization. “The dairy industry is at a watershed moment where sustainability and productivity must coexist,” explains Dr. Michael Niu, chief researcher at the ETH Zürich Department of Environmental Systems Science. Ready to embrace a more hopeful future for your farm’s production and environmental impact? Let’s dig in.

Balancing Act: Achieving High Milk Yields with Sustainable Practices in Modern Dairy Farming

One of the most challenging difficulties confronting dairy producers today is reconciling high milk output with the need for sustainability. It’s no longer simply about how much milk your herd can produce; the environmental impact of your enterprise is being closely scrutinized. Regulatory organizations enforce more muscular limitations for methane emissions and nitrogen excretion, encouraging farmers to adopt more environmentally friendly techniques. Meanwhile, customer demand for ecologically friendly dairy products is increasing, placing more pressure on farmers to innovate. The time to strike this balance is now, crucial not just for regulatory compliance and market competitiveness but also for the dairy industry’s long-term survival.

What is 3-NOP? 

3-Nitrooxypropanol, or 3-NOP, is an innovative feed additive used in dairy production to reduce methane emissions. But what does it accomplish, and why should you care? This additive, along with tannin extract, holds the potential to revolutionize dairy farming, reducing emissions and improving performance. It’s a game-changer, and it’s time to get on board.

When cows digest food, microorganisms in their rumen create methane, a potent greenhouse gas. 3-NOP comes into play here. It acts by blocking an enzyme required for methane synthesis in these microorganisms. To put it simply, 3-NOP reduces the effectiveness of methane-producing organisms.

Let us now discuss the positives. Reducing methane emissions benefits both the environment and your farm. Lower methane generation improves the overall efficiency of the cow’s digestive process, allowing more of the feed’s energy to flow into milk production instead of being wasted as gas. According to research, cows fed a 3-NOP-supplemented diet may lower methane emissions by 16% to 17% while maintaining milk output. This is not only excellent news for the environment, but it is also a reassuringly cost-effective solution. It may help you enhance the sustainability of your agricultural methods without breaking the bank.

Unlocking the Power of Tannins: A Game Changer for Dairy Farming 

Let’s discuss tannins, especially the extract from Acacia mearnsii. This extract has received a lot of interest in dairy farming because of its many advantages. Tannins are naturally occurring chemicals that bind and precipitate proteins. In dairy production, they are critical in nitrogen control.

One of the most noticeable impacts of tannins is their influence on nitrogen partitioning. When cows eat feed containing tannins, these chemicals may bind to proteins in their diet. This interaction lowers protein breakdown in the rumen while shifting nitrogen excretion from pee to feces. As a consequence, urinary nitrogen excretion has decreased by around 23.5%. This adjustment benefits the environment by reducing nitrogen’s contribution to groundwater pollution and greenhouse gas emissions.

Additionally, tannins in the diet have been shown to improve milk composition. Tannins, in particular, have been linked to higher levels of milk-accurate protein content and, in certain circumstances, yield. This not only benefits dairy producers but also meets consumer demand for high-protein dairy products. Furthermore, by enhancing nitrogen consumption inside the cow, tannins help to promote more sustainable and effective dairy production operations. This potential for improved milk quality should make you feel optimistic about the future of your product.

The ETH Zürich Study: Harnessing 3-NOP and Tannins for Optimal Dairy Cows Performance and Sustainability

The researchers at ETH Zürich investigated how the combination of 3-nitrooxypropanol (3-NOP) and Acacia mearnsii tannin extract (TAN) impacts lactational performance, methane emissions, and nitrogen partitioning in Brown Swiss and Holstein Friesian cattle. The experiment included sixteen cows, split evenly between Brown Swiss and Holstein Friesian breeds. Researchers used a split-plot design, dividing cows into a repeated 4 × 4 Latin square with a 2 x 2 factorial design across four 24-day periods.

Cows were fed four diets: a baseline total mixed ration (TMR), TMR with 3-NOP, TMR with TAN, and TMR with both 3-NOP and TAN. Milk output, methane emissions, and nitrogen excretion were among the measurements taken. The study found that TAN lowered milk urea nitrogen and urinary nitrogen without affecting milk output, but 3-NOP substantially reduced methane emissions across diets. Although no significant interaction between 3-NOP and TAN was found for any variable, the combination supplementation showed potential methane reduction and nitrogen management advantages.

Three Key Takeaways: 3-NOP, Tannins, and Their Synergy in Dairy Farming

The research presents three key results. First, 3-NOP decreased methane emissions by 16-17%, demonstrating its promise as a methane mitigator. Second, tannins reduced MUN concentration and urinary nitrogen by 23.5% without affecting milk output or efficiency. Finally, although there was no significant interaction between 3-NOP and tannins, their combination supplementation may provide a potential for methane reduction and enhanced nitrogen management in dairy cows.

The Breed Factor: Unearthing Varied Methane Reductions in Holstein Friesian vs. Brown Swiss Cows 

One of the most notable findings when investigating breed-specific impacts is the considerable difference in methane reduction between Holstein Friesian (HF) and Brown Swiss (BS) cows. The research found that methane emissions were significantly reduced in HF cows, with a 22% drop compared to a 13% reduction in BS cows. This divergence highlights the need to study breed-specific responses to nutritional treatments such as 3-NOP.

Why does this variation exist across breeds? While the research provides valuable information, it also raises essential problems requiring additional investigation. Physiological variations, digestive efficiency, and hereditary factors might all influence these results.

More study is needed to determine the underlying processes governing these breed-specific responses. This allows us to adapt mitigation methods better, ensuring that all breeds gain the most from these interventions. As we aim for sustainability in dairy farming, understanding and maximizing breed-specific impacts becomes more critical.

Practical Steps to Embrace 3-NOP and Tannins in Your Dairy Farm 

When contemplating using 3-NOP and tannin supplements in your dairy operations, practical actions may help you get the most significant outcomes. Consult a livestock nutritionist to determine the appropriate dose and mix for your herd’s requirements. 3-NOP at 60 mg/kg DM has been demonstrated to be helpful, whereas tannins may be injected at 3% DM. However, these numbers may need to be adjusted depending on your cows’ nutritional needs and current feed mix.

  • Integration into Existing Feeding Regimens:
    Incorporating these vitamins into your cows’ meals may be simple. To ensure equitable distribution, you may include 3-NOP straight into total mixed rations (TMR). Consider tannins from natural sources, such as Acacia mearnsii extract, which may be added to the diet. Ensure that the supplements are well-mixed to prevent selective feeding.
  • Monitoring and Adjustments:
    After you’ve introduced these vitamins, keep a watchful eye on your cows. Monitor feed intake, milk output, and general health. To determine the advantages, monitor methane emissions and nitrogen excretion. Use essential, accessible tools or work with academics for more sophisticated analysis.
  • Potential Challenges and Solutions:
    One problem may be the initial expense of incorporating supplements into your food routine. To mitigate this, the supplements should be introduced gradually, and the cost-benefit evaluated over time. Another possible concern is the heterogeneity in methane reduction among breeds. Address this by customizing dosages to breed-specific responses, beginning with the suggested quantities and modifying as data is gathered.

To summarize, including 3-NOP and tannins in your dairy business with appropriate planning and monitoring may result in long-term improvements. Despite the early obstacles, the potential for increased feed efficiency and lower methane emissions makes these supplements worthwhile. Consult with specialists, begin with trial stages, and keep adjusting for the best outcomes.

Frequently Asked Questions 

What are 3-NOP and tannins, exactly? 

3-NOP, or 3-Nitrooxypropanol, is a feed additive that decreases methane emissions from cows by blocking a critical methane-producing enzyme. Tannins, especially those derived from Acacia mearnsii, are plant chemicals that increase protein consumption in cow diets by binding to proteins and other nutrients in the rumen.

Are 3-NOP and tannins safe for my cows? 

Both 3-NOP and tannins are safe when used in the prescribed dosages. Extensive research, including a study by ETH Zürich, shows the safety and usefulness of these supplements in lowering methane emissions and improving nitrogen utilization while preserving milk supply.

Will these additives affect my cows’ milk production? 

No substantial detrimental influence on milk production has been detected. According to the research, tannin-fed cows produce the same amount of milk, possibly improving the accurate protein percentage. 3-NOP aims to reduce methane emissions, with no observed negative impacts on milk yields.

How much can I expect methane emissions to decrease? 

The research found that 3-NOP may cut methane emissions by 16% to 17%. Further decrease varies by breed, with Holstein Friesian cows exhibiting a 22% drop and Brown Swiss cows showing a 13% reduction. The combination of 3NOP with tannins provides additional environmental advantages.

What about other environmental impacts? 

Tannins reduce methane emissions while decreasing urinary nitrogen excretion by 23.5%, which may help reduce nitrogen pollution in the environment. This dual advantage contributes to more sustainable dairy production operations.

How do I integrate these additives into my cows’ diet? 

The study recommends adding 60 mg of 3-NOP per kg of dry matter (DM) and 3% tannin extract by DM to the total mixed ration (TMR). Appropriate dose and diet formulation are critical for the best outcomes. Consultation with a nutritionist or veterinarian may help you adjust these supplements to your herd’s requirements.

Are there cost implications? 

While the initial costs of acquiring these additives may be more significant, the long-term advantages, such as increased sustainability, improved nitrogen usage, and less environmental effect, often surpass the expenses. The improved operational efficiency and possibility for premium market positioning may potentially offer a financial offset.

Where can I source 3-NOP and tannin extracts? 

These chemicals are available from specialist agricultural suppliers and nutritional firms. Use high-quality, research-backed goods to guarantee safety and effectiveness. Consulting with industry professionals might also help you locate trustworthy suppliers.

Future Research: Unveiling Untapped Potentials and Answering Pressing Questions 

These results represent a big step toward sustainable dairy production but raise several issues for further study. One crucial need is to investigate the long-term effects of 3-NOP and tannin supplementation on cow health and production in different dairy breeds. While the study found differences between Holstein Friesian and Brown Swiss cows, further research might help determine the ideal breeds or genetic lines that respond well to these supplements.

Furthermore, understanding the processes driving differential methane decrease is critical. Why do Holstein Friesian cows produce less methane than Brown Swiss cows? Answering this question might lead to more focused and effective methane mitigation methods.

Another promising area for future study is determining the economic sustainability of broad deployment. While environmental advantages are vital, dairy producers must understand the costs and possible financial gains. Studies assessing cost-effectiveness and environmental benefits will be critical in building a compelling case for adoption.

Furthermore, combining 3-NOP and tannins with additional dietary supplements might provide even higher effects. Could there be a synergistic impact with other methane inhibitors or feed efficiency increases? These are questions that need investigation.

In the long run, combining 3-NOP and tannins might transform dairy production, making it more sustainable while maintaining productivity. Farmers who keep aware and adaptive will be at the vanguard of this shift, possibly benefiting both economically and environmentally.

Staying up to speed on new research and industry advancements is critical as we anticipate future investigations. Participating in the future of dairy farming has the potential to impact the industry significantly.

The Bottom Line

The combined use of 3-NOP and tannins represents a substantial advancement in dairy production. Using these supplements, you may reduce methane emissions by up to 17%, increase nitrogen usage, and refine milk quality indicators. Such advancements boost your herd’s production while promoting a more sustainable and environmentally friendly agricultural method.

Consider how 3-NOP and tannins might improve your dairy business. Are you prepared to move toward a more sustainable dairy farm?

Learn more:

Optimizing Dairy Cow Performance and Nitrogen Efficiency with Low-Protein, Red Clover, and Grass Silage Diets: The Role of Starch and Rumen-Protected Methionine Supplements

Discover how low-protein diets with red clover silage, supplemented with starch or rumen-protected methionine, can optimize dairy cow performance and nitrogen efficiency.

In the complex realm of dairy farming, the delicate balance between optimizing cow performance and nitrogen efficiency is the key to economic viability and environmental sustainability. A practical strategy that emerges is the reduction of dietary crude protein (CP) while incorporating nutrient-rich feeds like red clover and grass silage. This approach can significantly enhance milk production and mitigate nitrogen excretion, a major contributor to environmental pollution. By delving into the interplay of dietary protein levels and supplements such as starch or rumen-protected methionine (RPMet), this article provides practical insights into how these feed adjustments can drive performance and nitrogen use efficiency (NUE) in dairy cows. We explore the benefits and practical implications of low-protein, red clover, and grass silage-based diets, from maintaining milk yields and quality to reducing urinary nitrogen waste and improving apparent NUE.

The Advantages of Lowering Protein Intake in Dairy Cow Diets

Implementing a low-protein diet for dairy cows is beneficial for nitrogen efficiency, environmental impact, and milk production. 

  • Improved Nitrogen Efficiency: Low-CP diets enhance nitrogen use efficiency (NUE). Maintaining metabolizable protein (MP) supply while reducing CP content results in higher NUE percentages, optimizing metabolic processes and reducing nitrogen wastage.
  • Reduced Environmental Impact: Lower CP content decreases urinary nitrogen excretion, aiding in compliance with manure nitrogen regulations and reducing ammonia emissions, thus supporting sustainable agriculture.
  • Enhanced Milk Production: Despite lower protein content, milk yield and quality (fat, protein, lactose) remain stable, allowing for cost savings without compromising production efficiency or quality.

The Role of Red Clover and Grass Silage

Red clover and grass silage are essential in sustainable dairy cow diets. Red clover, a legume, fixes nitrogen, enhancing soil health and reducing the need for fertilizers. It is highly palatable and digestible, improving dairy cow performance. Red clover is rich in protein and fiber and supports rumen function and milk production. 

Grass silage complements red clover by providing a balanced forage that supports consistent intake and nutrient supply. Grass species like ryegrass have high sugar content, promoting better fermentation and increasing energy density. Red clover and grass silage together ensure a steady supply of energy and protein, which is not only essential for maximizing milk yield but also for maintaining cow health. This reassures us that these feed adjustments are not just about performance and efficiency, but also about the well-being of our cows. 

Integrating these silages into a total mixed ratio (TMR) offers a balanced dietary approach, ensuring each bite is nutritionally complete. This reduces selective feeding and improves overall nutrient intake, which is crucial for stable milk production and optimal nitrogen use efficiency (NUE), especially when adjusting crude protein (CP) levels. 

Our study refines dietary CP balance while maintaining metabolizable protein (MP) levels with supplements like starch or rumen-protected methionine (RPMet). This strategy aims to sustain and enhance performance metrics such as milk yield, composition, and NUE while reducing the environmental impact of dairy farming through lower nitrogen excretion.

Role of Red Clover in Dairy Cow Nutrition

Red clover plays a significant role in dairy cow nutrition, particularly enhancing nutrient digestibility. Research shows that its inclusion doesn’t significantly alter overall nutrient digestibility but helps maintain a balanced nutritional intake. This supports efficient digestion and metabolism in dairy cows. 

Regarding milk quality, red clover silage offers notable benefits. While our study found that milk yield and significant components like fat and protein remain unaffected by dietary CP content, there were essential changes in milk and plasma urea concentrations and fatty acid profiles. These findings suggest that red clover silage positively influences milk’s nutrient profile, benefiting both milk processors and consumers. This highlights the strategic value of incorporating red clover in dairy cow diets.

Advantages of Grass Silage in Dairy Cow Rations

Incorporating grass silage into dairy cow rations provides several key advantages. Its high fiber content promotes proper rumen function and efficient digestion, improving nutrient extraction—the fiber aids in producing volatile fatty acids, essential for the cow’s energy supply. 

Grass silage also supports rumen health. The fibrous structure fosters healthy microbial populations in the rumen, which is crucial for breaking down feed and absorbing nutrients. This can mitigate risks of metabolic disorders like acidosis, which are familiar with low-fiber diets. 

Economically, grass silage is a cost-effective forage. It often requires fewer inputs than other forage crops, making it affordable for many dairy farmers. It can be grown in various soil types and climates, usually needing less fertilizer and pesticides while still providing adequate energy and protein for milk production.

Understanding Crude Protein: Why Less is More

Reducing dietary crude protein (CP) can cut costs and lessen milk production’s environmental impact. As high-protein diets become more costly and regulations on nitrogen emissions tighten, this is more relevant than ever. This study examines the benefits of lowering CP levels in red clover silage—a valuable but underutilized resource. 

Reducing CP goes beyond cost savings. Environmentally, it lowers ammonia emissions and urinary nitrogen excretion. Our study found that cutting CP from 175 g/kg DM to 150 g/kg DM improved nitrogen use efficiency (NUE) without compromising dairy performance, meeting global sustainability goals

Cows on low-protein (LP) diets with additional starch (LPSt) or rumen-protected methionine (LPM) maintained consistent milk yields and nutrient digestibility. This dispels myths about performance declines with lower protein intake. By ensuring adequate metabolizable protein (MP), producers can sustain optimal performance and reduce environmental harm. 

Milk fat, protein, and lactose levels were stable across diets, suggesting no compromise in milk quality. Plasma urea and β-hydroxybutyrate concentrations also showed the body’s adaptive responses to reduced protein intake. 

These results suggest a shift in dairy nutrition toward economic efficiency, environmental responsibility, and maintenance performance. Dairy producers can better meet modern farming challenges by using red clover silage with strategic protein reduction and supplementation.

Starch and Rumen-Protected Methionine: Key Supplements Explained

Starch and rumen-protected methionine (RPMet) enhance dairy cow diets’ nutritional profile and metabolic efficiency, especially legume silages like red clover. Starch from grains such as barley boosts energy, supporting microbial protein synthesis in the rumen, thus aiding milk production. It offers quick energy, which is crucial for peak lactation and high-energy demands. 

Methionine is an essential amino acid critical for protein synthesis and metabolic functions. Rumen-protected methionine bypasses rumen degradation, reaching the small intestine intact for effective absorption and aiding milk protein synthesis and quality. 

While supplementing low-protein diets with starch or RPMet theoretically offsets reduced crude protein levels, the study revealed no significant impact on overall milk yield or composition. However, RPMet supplementation altered metabolic parameters, increasing blood plasma β-hydroxybutyrate levels. Conversely, the LPSt diet reduced plasma urea concentrations, suggesting improved nitrogen utilization. 

These findings highlight that starch and RPMet fine-tune dietary balance, but their broader metabolic effects are crucial. Increased nitrogen use efficiency (NUE) across all low-CP diets indicates a sustainable approach to dairy nutrition, reducing nitrogen excretion and environmental impact without compromising performance.

Comparing Dietary Treatments: Control vs. Low-Protein Diets

ParameterControl (CON)Low-Protein (LP)LP + Starch (LPSt)LP + Rumen-Protected Methionine (LPM)
Dry Matter Intake (DMI) kg/d21.521.521.521.5
Milk Yield (kg/d)UnalteredUnalteredUnalteredUnaltered
Milk Urea ConcentrationHighestLowerLowerLower
Plasma β-Hydroxybutyrate LevelsLowestHighest
Apparent Nitrogen Use Efficiency (NUE)28.6%34.2%34.2%34.2%
Urinary Nitrogen Excretion (g/d)Higher~60 g/d Lower~60 g/d Lower~60 g/d Lower

In comparing the control diet (CON) with 175 g/kg DM of crude protein against the low-protein diets (LP, LPSt, and LPM) at 150 g/kg DM, we found no notable difference in dry matter intake (DMI), which averaged 21.5 kg/day across all diets. DMI did vary by week and diet, peaking in the LPSt diet during week four and in the CON diet during weeks 9 and 14. 

Milk yield, energy-corrected milk (ECM), and 4% fat-corrected milk (FCM) were consistent across all treatments, suggesting a lower CP content did not affect overall milk production. Milk composition, including fat, protein, and lactose, remained stable. However, cows on the CON diet had higher milk and plasma urea levels, indicating excess nitrogen intake. 

The blood plasma β-hydroxybutyrate levels varied, highest in the LPM diet and lowest in the LPSt diet. Improved nitrogen use efficiency (NUE) was observed in cows on low-protein diets, with an NUE of 34.2% compared to 28.6% in the control group. This shows the efficiency and environmental benefits of low-protein diets. 

Nutrient digestibility, measured as the digestibility of organic matter, nitrogen, neutral detergent fiber (NDF), and acid detergent fiber (ADF), showed no significant differences across treatments. Yet, urinary nitrogen excretion was reduced by about 60 g/day in cows on low-CP diets, highlighting the environmental and economic advantages of lowering dietary CP without compromising animal performance.

The Bottom Line

Optimizing dairy cow performance while enhancing nitrogen use efficiency offers a dual benefit: sustainable milk production and reduced environmental impact. Dairy farmers can maintain milk yield and quality by adjusting crude protein levels with red clover and grass silage without compromising herd well-being. 

Our analysis highlights the benefits of grass silage, the importance of maintaining adequate metabolizable protein (MP), and the roles of supplements like starch and rumen-protected methionine (RPMet). Reducing CP content from 175 to 150 g/kg DM leads to higher nitrogen efficiency (NUE) and lower urinary nitrogen excretion. 

Adopting low-protein diets with red clover and grass silage is a promising strategy for dairy farmers focused on productivity and environmental regulations. Our findings show that these dietary adjustments do not hinder performance but promote sustainability. Consider integrating low-protein, red clover, and grass silage into your dairy cows’ diet to enhance nitrogen efficiency and overall herd performance.

Key Takeaways:

  • Reducing dietary crude protein (CP) from 175 g/kg DM to 150 g/kg DM in red clover and grass silage-based diets, while maintaining metabolizable protein (MP) supply, does not compromise dairy cow performance.
  • Supplementation with dietary starch or rumen-protected methionine (RPMet) in low-CP diets had limited impact on overall milk yield and composition.
  • Cows on low-CP diets exhibited improved nitrogen use efficiency (NUE), with higher mean NUE values compared to those on standard CP diets.
  • Milk and plasma urea concentrations were significantly lower in cows fed low-CP diets, indicating better protein utilization and reduced nitrogen wastage.
  • Lower CP diets resulted in reduced urinary nitrogen excretion by approximately 60 g/d, supporting environmental sustainability and compliance with manure nitrogen regulations.
  • The overall apparent nutrient digestibility remained consistent across different dietary treatments, suggesting that performance metrics are maintained despite reduced CP levels.
  • Economic viability of milk production may be enhanced by reducing protein intake without sacrificing production efficiency or milk quality.

Summary: The balance between optimizing cow performance and nitrogen efficiency is crucial for economic viability and environmental sustainability in dairy farming. A practical strategy is reducing dietary crude protein (CP) while incorporating nutrient-rich feeds like red clover and grass silage. This approach can significantly enhance milk production and mitigate nitrogen excretion, a major contributor to environmental pollution. Low-protein diets enhance nitrogen use efficiency (NUE), maintain metabolizable protein (MP) supply, and reduce nitrogen wastage. Lower CP content decreases urinary nitrogen excretion, aiding in compliance with manure nitrogen regulations and reducing ammonia emissions, thus supporting sustainable agriculture. Enhanced milk production remains stable, allowing for cost savings without compromising production efficiency or quality. Red clover plays a significant role in dairy cow nutrition, particularly enhancing nutrient digestibility. Grass silage in dairy cow rations provides several advantages, such as high fiber content, proper rumen function, efficient digestion, and economic affordability. This study explores the benefits of reducing dietary crude protein in red clover silage, a valuable but underutilized resource. Reducing CP goes beyond cost savings and environmentally lowers ammonia emissions and urinary nitrogen excretion. Supplementing low-protein diets with starch or rumen-protected methionine (RPMet) theoretically offsets reduced crude protein levels, but no significant impact on overall milk yield or composition was found.

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